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About This Simulation
Become an environmental investigator and solve a massive fish kill mystery. Use a spectrophotometer to analyze oxygen levels in the water and unravel the mystery.
Learning Objectives
- Understand the basic concepts of sample collection
- Analyze the level of dissolved oxygen level in your water sample using a spectrophotometer
- Learn the concepts of a calibration curve, linear regression and extrapolation
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About This Simulation
Lab Techniques
- Spectrophotometry
- Sampling
Related Standards
- No direct alignment
- Environmental Science 1.3 Aquatic biomes
- Environmental Science 8.6 Thermal pollution
- Biology Topic 5: Evolution and biodiversity
Learn More About This Simulation
This short, targeted simulation is adapted from the full-length “Marine Biology” simulation.
Can you get to the bottom of a mysterious massive fish-killing? In this simulation, you will be an environmental investigator to solve this fish kill mystery. You will analyse the oxygen levels in the water using a spectrophotometer and discover the impact of the oxygen levels on the fish.
The mass fish-killing
Meet Trisha at the bay where you learn of a mass fish killing. Make a hypothesis about what happened and discuss how you could find the cause of the fish killing. Take samples from the site of the fish killing before returning to the lab.
Analyze the oxygen levels in water
You collected water samples from the bay where the fish were found. Start your analysis by learning about the principles of spectrophotometry. To measure the absorbance of your samples you need to add a coloring agent that has more intense color if more oxygen is in the sample. Use a spectrophotometer to measure the absorbance of the water sample and samples containing a known amount of oxygen. Plot a calibration curve to compare the dissolved oxygen levels in the water sample to the known value samples to investigate whether low oxygen in the lake could have caused the massive fish death. Do the results of this analysis correspond with the symptoms exhibited by the fish?
Solve the mystery
After gathering evidence by analysing the dissolved oxygen levels in the water samples, you will need to figure out what caused the massive fish death. Will you be able to solve the mystery?
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For Science Programs Providing a Learning Advantage
North Dakota State College of Science
“They did the simulation at home, then completed the in-person lab within 30 minutes, no questions asked, and passed the quiz with flying colors.”
Lecturer in Human Physiology
University of Westminster
"I saw some of the students who clearly didn’t necessarily like sitting there reading a book discover they could turn on Labster and keep up with the rest of the class because it spoke to them.
Modesto City Schools
"Having something that's engaging for the students gives teachers that opportunity to breathe and get excited again. Because they're seeing the kids light up, they're seeing the kids engage with content."
Wenatchee Valley College
"The question always is, ‘Can we demonstrate that the students are meeting course outcomes?’ Check! We can do that.”
San José State University
"We surveyed over 400 students. More than 90% thought Labster was easy to navigate, and that it was fun, but more importantly, most of them felt confident that they could execute the labs in person. And that confidence is a big deal."
FAQs
Find answers to frequently asked questions.
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