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About This Simulation
Join cutting edge research at MIT and design a biological circuit that can sense and destroy cancer cells.
Learning Objectives
- Engineer natural systems to perform specific functions
- Describe the fundamentals of the Gateway cloning technique and design your own biological circuit
- Explain and perform bacterial transformation, antibiotic selection and plasmid purification
- Explain and perform a restriction digest of your cloning product
About This Simulation
Lab Techniques
- Gel electrophoresis
- Restriction digest
- Electroporation
- Antibiotics selection
- Plasmid isolation with purification columns
- Gateway cloning technique
- Sterile technique
Related Standards
- No direct alignment
- No direct alignment
- No direct alignment
Learn More About This Simulation
Use the specific microRNA profile of cancer cells to design an apoptotic biological circuit that is only activated in cancer cells. You are the only hope for a patient with a rare form of cancer.
Learn how to use the Gateway cloning technique
In the Synthetic Biology simulation, you will learn how to use the Gateway cloning technique to combine different genetic modules in an expression vector. You will learn how the Gateway cloning technique can be used to efficiently combine these modules. Use electroporation to transform the bacteria with your vector of interest and select successful transformants. You will learn all about the basics of sterile lab work and bacterial selection.
Extract and isolate plasmids
Your next task in the lab will be to grow the transformed bacteria and extract the plasmids using a ‘miniprep’ kit. You will learn how different buffers enable you to isolate plasmid, and not genomic, DNA from the cells. You will check if the plasmid, indeed, contains your circuit, and if it is mutation-free by digesting it with restriction enzymes and by using gel electrophoresis. In the end you will test your circuit in living cells to see if the cancer cells are the only cells that die.
Will you be able to find a cure for this rare form of cancer?
Experience Labster for Yourself
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For Science Programs Providing a Learning Advantage
North Dakota State College of Science
“They did the simulation at home, then completed the in-person lab within 30 minutes, no questions asked, and passed the quiz with flying colors.”
Lecturer in Human Physiology
University of Westminster
"I saw some of the students who clearly didn’t necessarily like sitting there reading a book discover they could turn on Labster and keep up with the rest of the class because it spoke to them.
Modesto City Schools
"Having something that's engaging for the students gives teachers that opportunity to breathe and get excited again. Because they're seeing the kids light up, they're seeing the kids engage with content."
Wenatchee Valley College
"The question always is, ‘Can we demonstrate that the students are meeting course outcomes?’ Check! We can do that.”
San José State University
"We surveyed over 400 students. More than 90% thought Labster was easy to navigate, and that it was fun, but more importantly, most of them felt confident that they could execute the labs in person. And that confidence is a big deal."
FAQs
Find answers to frequently asked questions.
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Labster can be integrated within a school's LMS (Learning Management System), and students can access it like any other assignment in their LMS. If your Institution does not choose an LMS integration, students will log in to Labster's Course Manager once they have an account created. Your institution will decide the access method during the sales process.
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Labster simulations are created by real scientists and designed with unparalleled interactivity. Unlike point and click competitors, Labster simulations immerse students and encourage mastery through active learning.
Labster supports a wide range of courses at the high school and university level across fields in biology, chemistry and physics. Some simulations mimic lab procedures with high fidelity to train foundational skills, while others are meant to bring theory to life through interactive scenarios.