Heading 1
Heading 2
Heading 3
Heading 4
Heading 5
Heading 6
Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur.
Block quote
Ordered list
- Item 1
- Item 2
- Item 3
Unordered list
- Item A
- Item B
- Item C
Bold text
Emphasis
Superscript
Subscript
About This Simulation
Learn how to detect the hallmarks of EMT using immunofluorescence and knock out the correct gene to revert the process using CRISPR
Learning Objectives
- Set up a protocol to get answers to a biological question related to the EMT process
- Acknowledge mistakes in the protocols and understand how to critically overcome them
- Properly prepare cells for the immunostaining procedure according to the localization of the target protein
- Select a suitable secondary antibody for an immunofluorescence experiment
- Analyze immunofluorescence results
- Describe the basics of CRISPR-Cas technique
- Design a guide RNA construct for knock-out strategies
- Evaluate CRISPR-Cas9 results
About This Simulation
Lab Techniques
- CRISPR-Cas9
- Immunofluorescence
Related Standards
- No direct alignment
- No direct alignment
- No direct alignment
Learn More About This Simulation
CRISPR may sound like a brand of cereals, but it certainly isn’t! In this simulation, you will learn to identify the molecular hallmarks of the Epithelial-to-Mesenchymal Transition (EMT) molecular process in breast cancer cells. You will induce EMT by adding TGF-beta to the cells, and, after identifying the hallmarks with immunofluorescence labeling, you will use the cutting-edge CRISPR-Cas9 technology to try to revert this process, which is one of the main causes of cancer malignancy.
Set up your own immunofluorescence protocol
In your first mission, you will be able to perform an immunostaining protocol by making decisions on the key steps of the process. Each decision can get you closer to the correct outcome, so think carefully and choose wisely!
Use CRISPR to knock out a specific gene
Once you have learned what the molecular hallmarks of EMT are, you will be able to dive into a eukaryotic cell to learn how the CRISPR-Cas9 technique works at a molecular level. Then, you will learn to design one of the CRISPR-Cas9 components by solving a fun puzzle. Last, you will select the target gene to knock out and your virtual lab assistant, Dr. One, will help you with the last part of the immunostaining process so you can directly evaluate your results.
Repeat your experiment as many times as you want
At the end of each mission, you will be able to repeat the experiment if the outcome was not the desired one, giving you the possibility of learning from your mistakes. Luckily, Dr. One will be there to help you with some of the tasks. In the end, you will be able to complete an experiment that takes weeks in just less than one hour!
Will you be able to set the right protocols in place, solving any issues that arise, to understand the molecular changes linked to the TGF-beta induced EMT and evaluate the possibility of using CRISPR-Cas9 as a possible therapeutic approach in breast cancer?
Experience Labster for Yourself
Boost Learning with Fun
75% of students show high engagement and improved grades with Labster
Discover Simulations That Match Your Syllabus
Easily bolster your learning objectives with relevant, interactive content
Place Students in the Shoes of Real Scientists
Practice a lab procedure or visualize theory through narrative-driven scenarios
Related Course
+20 other courses
Protein Synthesis
Explore the structure of proteins and learn about the synthesis process inside the cells. Examine the protein sequence to understand the differences of protein synthesis in prokaryotes and eukaryotes.
Bacterial Cell Structures: An introduction to the bacterial cell
Visit a research station in Antarctica and help the researcher Nicolas explore bacteria in melting water. Uncover the features that are necessary for bacterial survival and compare these to other bacteria living elsewhere.
Nucleophilic Substitution Reaction: Alkyl halides substrates
Solve a craft beer conundrum using your substitution smarts to design reactions for a unique range of flavor molecules! Will you help draw in a hip crowd with the new beverage flavors you create in the brew lab?
For Science Programs Providing a Learning Advantage
North Dakota State College of Science
“They did the simulation at home, then completed the in-person lab within 30 minutes, no questions asked, and passed the quiz with flying colors.”
Lecturer in Human Physiology
University of Westminster
"I saw some of the students who clearly didn’t necessarily like sitting there reading a book discover they could turn on Labster and keep up with the rest of the class because it spoke to them.
Modesto City Schools
"Having something that's engaging for the students gives teachers that opportunity to breathe and get excited again. Because they're seeing the kids light up, they're seeing the kids engage with content."
Wenatchee Valley College
"The question always is, ‘Can we demonstrate that the students are meeting course outcomes?’ Check! We can do that.”
San José State University
"We surveyed over 400 students. More than 90% thought Labster was easy to navigate, and that it was fun, but more importantly, most of them felt confident that they could execute the labs in person. And that confidence is a big deal."
FAQs
Find answers to frequently asked questions.
Heading 1
Heading 2
Heading 3
Heading 4
Heading 5
Heading 6
Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur.
Block quote
Ordered list
- Item 1
- Item 2
- Item 3
Unordered list
- Item A
- Item B
- Item C
Bold text
Emphasis
Superscript
Subscript
Labster can be integrated within a school's LMS (Learning Management System), and students can access it like any other assignment in their LMS. If your Institution does not choose an LMS integration, students will log in to Labster's Course Manager once they have an account created. Your institution will decide the access method during the sales process.
Labster is only available for purchase by faculty and administration at academic institutions. To procure Labster, simply reach out to us on our website. Schedule a demo, book a meeting to discuss pricing, start a free trial, or simply fill out our contact form.
Labster simulations are created by real scientists and designed with unparalleled interactivity. Unlike point and click competitors, Labster simulations immerse students and encourage mastery through active learning.
Labster supports a wide range of courses at the high school and university level across fields in biology, chemistry and physics. Some simulations mimic lab procedures with high fidelity to train foundational skills, while others are meant to bring theory to life through interactive scenarios.