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About This Simulation
Use liquid chromatography to purify a protein involved in Parkinson’s disease to learn more about its causes and evaluate the efficacy of a promising molecule for its treatment using confocal microscopy.
Learning Objectives
- Describe the role of alpha synuclein in Parkinson’s disease
- Describe the different parts of a liquid chromatography equipment
- Interpret results from liquid chromatography experiments
- Describe the difference between Ion Exchange Chromatography and Size Exclusion Chromatography
- Evaluate the mode of action of Epigallocatechin gallate on Lewy body formation
- Describe the benefits of using Large Unilamellar vesicles as a cell membrane in vitro model
About This Simulation
Lab Techniques
- Confocal microscopy
- Sonication
- Size exclusion chromatography
- Ion exchange chromatography
Related Standards
- No direct alignment
- No direct alignment
- No direct alignment
Learn More About This Simulation
Your grandmother has recently been diagnosed with Parkinson’s disease. In this simulation, you will investigate the effects of a molecule found in green tea on the degeneration of neurons using an in vitro model.
Purifying alpha synuclein monomers
You will extract and purify monomers of alpha synuclein (aSN), a protein that is involved in the formation of Lewy bodies in the brain during the development of Parkinson’s disease. In order to do so, you will first use sonication to extract the protein, which is produced by an E. coli bacterial strain, and which was previously genetically modified by your lab assistant. Then, you will purify the extracted aSN monomers by ion exchange chromatography, while you learn about the different parts of the liquid chromatography equipment to get familiar with them before operating the equipment in the real lab.
Inducing and purifying aSN oligomers
After purifying the aSN monomers, you will induce the formation of aSN oligomers using a simple heating procedure. Once the oligomers are formed, you will further purify the sample to separate aSN monomers from oligomers using size exclusion chromatography. You don’t need to worry about long time-consuming preparation steps, because time will be fast-forwarded so you can focus on evaluating the results!
Evaluation of EGCG therapeutic potential
At the end of the Parkinson’s disease simulation, you will use the purified aSN monomers and oligomers to test the potential effects of Epigallocatechin gallate (EGCG), a polyphenol from green tea. You will use Large Unilamellar Vesicles containing a fluorescent dye as the membrane model to reproduce the in vivo conditions, and evaluate your results using confocal microscopy. Finally, you will take a trip inside the sample to find out the molecular mechanism that causes neurons to degenerate.
Will you be able to elucidate the mechanism of action of EGCG to provide your grandmother with insights about the benefits of green tea?
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For Science Programs Providing a Learning Advantage
North Dakota State College of Science
“They did the simulation at home, then completed the in-person lab within 30 minutes, no questions asked, and passed the quiz with flying colors.”
Lecturer in Human Physiology
University of Westminster
"I saw some of the students who clearly didn’t necessarily like sitting there reading a book discover they could turn on Labster and keep up with the rest of the class because it spoke to them.
Modesto City Schools
"Having something that's engaging for the students gives teachers that opportunity to breathe and get excited again. Because they're seeing the kids light up, they're seeing the kids engage with content."
Wenatchee Valley College
"The question always is, ‘Can we demonstrate that the students are meeting course outcomes?’ Check! We can do that.”
San José State University
"We surveyed over 400 students. More than 90% thought Labster was easy to navigate, and that it was fun, but more importantly, most of them felt confident that they could execute the labs in person. And that confidence is a big deal."
FAQs
Find answers to frequently asked questions.
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Labster can be integrated within a school's LMS (Learning Management System), and students can access it like any other assignment in their LMS. If your Institution does not choose an LMS integration, students will log in to Labster's Course Manager once they have an account created. Your institution will decide the access method during the sales process.
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Labster simulations are created by real scientists and designed with unparalleled interactivity. Unlike point and click competitors, Labster simulations immerse students and encourage mastery through active learning.
Labster supports a wide range of courses at the high school and university level across fields in biology, chemistry and physics. Some simulations mimic lab procedures with high fidelity to train foundational skills, while others are meant to bring theory to life through interactive scenarios.