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About This Simulation
Learn how different factors such as heat and humidity can alter drug stability. Identify the components of the HPLC machine and use it to separate and measure the different compounds of a medicine.
Learning Objectives
- Understand the different compartments of an HPLC machine and functions
- Understand the principles of HPLC separation
- Understand the lipophilic interaction between the analyte and the mobile and stationary phase
- Understand the different changes in parameters (such as the column, mobile phase, temperature etc.) and its effects on analyte separation and concentration measurements
About This Simulation
Lab Techniques
- HPLC
Related Standards
- HS-PS1-3, HS-PS2-6
- 3.9 Separation of Solutions and Mixtures Chromatography
- B.9 Biological Pigments
Learn More About This Simulation
Explore the HPLC machine by taking a look inside it. Find the different components including the sample injection station, column, pumps and UV detector, and learn all about the principles of liquid chromatography, specifically HPLC.
Understanding High Performance Liquid Chromatography
In the HPLC simulation, your first task is to select different mobile phases and different columns to run your experiment. You will then separate the components of a drug and measure concentrations. Next, compare the separation profile using different mobile phases and columns. During the experiment, you will be able to visualize the lipophilic interaction between analyte and both the stationary and mobile phase by watching a 3D animation. Quiz questions throughout the lab check will check your understanding of the HPLC technique.
Drug stability analysis
After completing the first part of the HPLC lab, you will learn how to analyze drugs that have been stored in different conditions and measure and compare the active ingredient’s concentration.
About the HPLC lab
The HPLC Lab is constructed with specific detail and information that can be beneficial for anyone pursuing pharmaceutical studies. Additionally, it can be applied to any area of study that uses chromatography techniques such as biology and biochemistry. Chromatography is an important technique used by pharmacists, and drug stability is an important issue that a pharmacist must be aware of. This lab combines the technique and application.
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For Science Programs Providing a Learning Advantage
North Dakota State College of Science
“They did the simulation at home, then completed the in-person lab within 30 minutes, no questions asked, and passed the quiz with flying colors.”
Lecturer in Human Physiology
University of Westminster
"I saw some of the students who clearly didn’t necessarily like sitting there reading a book discover they could turn on Labster and keep up with the rest of the class because it spoke to them.
Modesto City Schools
"Having something that's engaging for the students gives teachers that opportunity to breathe and get excited again. Because they're seeing the kids light up, they're seeing the kids engage with content."
Wenatchee Valley College
"The question always is, ‘Can we demonstrate that the students are meeting course outcomes?’ Check! We can do that.”
San José State University
"We surveyed over 400 students. More than 90% thought Labster was easy to navigate, and that it was fun, but more importantly, most of them felt confident that they could execute the labs in person. And that confidence is a big deal."
FAQs
Find answers to frequently asked questions.
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Labster supports a wide range of courses at the high school and university level across fields in biology, chemistry and physics. Some simulations mimic lab procedures with high fidelity to train foundational skills, while others are meant to bring theory to life through interactive scenarios.