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About This Simulation
Let’s awaken your curiosity for the chemistry of caffeine. Learn how Flow Injection Analysis (FIA) helps scientists measure the concentration of caffeine in your drinks.
Learning Objectives
- Measure caffeine concentration in different samples using the Flow Injection Analysis (FIA) technique
- Describe the importance of the different parts of the FIA machine (e.g. the spectrophotometer, the pump, or the mixing coil)
- Describe the advantages of the FIA method
- Calculate the velocity at which a sample will be eluted
- Prepare sample dilutions and standard curves
- Interpret the results from a FIA experiment
- Determine the concentration of an unknown caffein sample by using the standard curve
About This Simulation
Lab Techniques
- Flow Injection Analysis
Related Standards
- HS-PS1-3, HS-PS2-6
- 3.9 Separation of Solutions and Mixtures Chromatography
- B.9 Biological Pigments
Learn More About This Simulation
Lego blocks and caffeine make an interesting combo. In this simulation, you will learn that a couple of scientists actually used lego blocks to build the first version of the Flow Injection Analysis (FIA) machine. You’ll use the technique to measure caffeine levels in various beverages.
Explore the parts of the flow injection machine
Not every real-life lab has a FIA machine, and it’s definitely not everyday that you get to look inside one. So in this simulation, you’ll get to explore the different parts of the FIA machine in detail through a 3D animation that illustrates the main mechanisms of the machine. You’ll soon understand how the machine detects caffeine, and apply this knowledge to your experiments.
Optimize and perform the experiment
Before you carry out an experiment, you will have to optimize the FIA machine for use. You can alter different machine parameters, to help generate the most accurate results. Once you have optimized the machine and prepared samples, you are ready to perform your experiment! Lots of beverages are waiting to be tested.
Compute the caffeine concentration
When you have finished your experiment, you’ll want to present your results in the best way possible. After creating an absorbance graph, you’ll employ your stoichiometry knowledge to determine the caffeine concentration of each beverage.
Maybe coffee is not the best drink for getting up or staying awake after all… maybe it’s hot chocolate! Will you be able to find out which beverages will cure you from sleepiness?
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For Science Programs Providing a Learning Advantage
North Dakota State College of Science
“They did the simulation at home, then completed the in-person lab within 30 minutes, no questions asked, and passed the quiz with flying colors.”
Lecturer in Human Physiology
University of Westminster
"I saw some of the students who clearly didn’t necessarily like sitting there reading a book discover they could turn on Labster and keep up with the rest of the class because it spoke to them.
Modesto City Schools
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Wenatchee Valley College
"The question always is, ‘Can we demonstrate that the students are meeting course outcomes?’ Check! We can do that.”
San José State University
"We surveyed over 400 students. More than 90% thought Labster was easy to navigate, and that it was fun, but more importantly, most of them felt confident that they could execute the labs in person. And that confidence is a big deal."
FAQs
Find answers to frequently asked questions.
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Labster can be integrated within a school's LMS (Learning Management System), and students can access it like any other assignment in their LMS. If your Institution does not choose an LMS integration, students will log in to Labster's Course Manager once they have an account created. Your institution will decide the access method during the sales process.
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Labster simulations are created by real scientists and designed with unparalleled interactivity. Unlike point and click competitors, Labster simulations immerse students and encourage mastery through active learning.
Labster supports a wide range of courses at the high school and university level across fields in biology, chemistry and physics. Some simulations mimic lab procedures with high fidelity to train foundational skills, while others are meant to bring theory to life through interactive scenarios.