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About This Simulation
Perform the chromatin immunoprecipitation with exonuclease treatment (ChIP-exo) protocol, and prepare the target DNA to learn how E. coli survive in acid stress conditions.
Learning Objectives
- Understand the use of exonuclease treatment in ChIP-exo technique.
- Understand the key steps for performing the ChIP-exo technique as well as the importance of each reagent:
- Adding formaldehyde to the culture to fix the bonds between proteins and DNA.
- Lysing the cells using lysozyme and sonication.
- Addition of specific antibodies.
- Use of magnetic beads.
- On-bead enzymatic modifications.
- Off-bead enzymatic modifications.
- Understand the molecular mechanism of ChIP-exo.
About This Simulation
Lab Techniques
- DNA editing
- PCR
- Immunodetection
- Sonication
- Cell lysis
- Immunoprecipitation
Related Standards
- No direct alignment
- No direct alignment
- No direct alignment
Learn More About This Simulation
In the ChIP-exo simulation, you will perform the chromatin immunoprecipitation with exonuclease treatment (ChIP-exo) protocol in order to get a DNA sequence to elucidate the acid stress mechanism of Escherichia coli. You will learn how to fix the protein-DNA interactions and lyse the cells using a sonicator, add magnetic beads to the sample, and perform different on-bead and off-bead enzymatic modifications. From this, you will learn how the exonucleases work.
Will you be able to prepare the target DNA to understand how E. coli survive in acid stress conditions?
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For Science Programs Providing a Learning Advantage
North Dakota State College of Science
“They did the simulation at home, then completed the in-person lab within 30 minutes, no questions asked, and passed the quiz with flying colors.”
Lecturer in Human Physiology
University of Westminster
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Modesto City Schools
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Wenatchee Valley College
"The question always is, ‘Can we demonstrate that the students are meeting course outcomes?’ Check! We can do that.”
San José State University
"We surveyed over 400 students. More than 90% thought Labster was easy to navigate, and that it was fun, but more importantly, most of them felt confident that they could execute the labs in person. And that confidence is a big deal."
FAQs
Find answers to frequently asked questions.
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Labster can be integrated within a school's LMS (Learning Management System), and students can access it like any other assignment in their LMS. If your Institution does not choose an LMS integration, students will log in to Labster's Course Manager once they have an account created. Your institution will decide the access method during the sales process.
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Labster supports a wide range of courses at the high school and university level across fields in biology, chemistry and physics. Some simulations mimic lab procedures with high fidelity to train foundational skills, while others are meant to bring theory to life through interactive scenarios.